Influence of the Interaction between p53 and ZNF568 on Mitochondrial Oxidative Phosphorylation
The tumor suppressor p53 plays important roles in suppressing the development and progression of cancer by responding to various stress signals. In addition, p53 can regulate the metabolic pathways of cancer cells by regulating energy metabolism and oxidative phosphorylation. Here, we present a mechanism for the interaction between p53 and ZNF568. Initially, we used X-ray crystallography to determine the irregular loop structure of the ZNF568 KRAB domain; this loop plays an important role in the interaction between p53 and ZNF568. In addition, Cryo-EM was used to examine how the p53 DBD and ZNF568 KRAB domains bind together. The function of ZNF568 on p53-mediated mitochondrial respiration was confirmed by measuring glucose consumption and lactate production. These findings show that ZNF568 can reduce p53-mediated mitochondrial respiratory activity by binding to p53 and inhibiting the transcription of SCO2.
* Reference
- Authors(Pusan National University): Dr. Chang Woo Han(Institute of Systems Biology), Prof. Se Bok Jang(Department of Molecular Biology)
- Title of original paper: Influence of the Interaction between p53 and ZNF568 on Mitochondrial Oxidative Phosphorylation
- Journal: International Journal of Biological Macromolecules
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